ABSTRACT
Se presenta un estudio retrospectivo de 122 pacientes con tumores que se manifestaron en la cavidad oral, con localización inicial en maxilares o partes blandas (se excluyeron los tumores de la cara sin compromiso bucal). La edad promedio fue 9 años y 6 meses (rango de 1 día a 17 años). El 62 por ciento se presentó en varones. La localización inicial de los tumores fue en hueso en el 53 por ciento de los casos y en partes blandas en el 47 por ciento; 82 pacientes tuvieron lesiones benignas y 40 lesiones malignas. Las manifestaciones al ingreso fueron: tumor palpable o visible (39 por ciento), tumor más dolor (22 por ciento), dolor (19 por ciento) y otros como caída de dientes, parálisis, fiebre o asímetría facial (20 por ciento). La rutina de estudio comprendió radiografía panorámica de maxilar, centellografía ósea (gammacámara con Tecnesio 99), tomografía axial computada (TAC) y resonancia nuclear magnética (RNM). Los pacientes fueron tratados en forma multidisciplinaria siendo la cirugía (punción aspiración con aguja fina, biopsia y/o resección)el procedimiento inicial en la mayoría de ellos. De acuerdo al algoritmo todos los pacientes con lesión ósea fueron estudiados con Rx simple y TAC, 89 por ciento de positividad en ambas, previas a la biopsia por punción. De igual menera en los tumores de partes blandas la TAC mantuvo su utilidad, no así la Rx simple que fue reeplazada por la ecografía cuando se detectó ausencia de compromiso óseo. Las lesiones benignas predominaron (78/122) a nivel de hueso o de partes blandas. la curación en ella fue la regla. En lo que respecta a los tumores, primarios de la región (11/40) correspondieron inicialmente a partes blandas y raramente a hueso, en los que fue frecuente el compromiso metastático o multicéntrico.
Subject(s)
Humans , Child , Mouth/surgery , Mouth , Retrospective Studies , Mouth Neoplasms , Mouth Neoplasms/diagnosisABSTRACT
Susceptibility to experimental autoimmune uveitis (EAU) in inbred mice has been associated with a dominant Th1 response. Elevated anti-inter-photoreceptor retinoid-binding protein (anti-IRBP) IgG2a/IgG1 antibody ratios have been implicated as candidate markers to predict disease severity. In the present study, both the anti-IRBP antibody isotype and severity of EAU phenotypes were examined in 4 non-isogenic genetically selected mouse lines to determine if they can be used as general markers of disease. Mice between 8 and 12 weeks old selected for high (H III) or low (L III) antibody response and for maximum (AIR MAX) or minimum (AIR MIN) acute inflammatory reaction (AIR) were immunized with IRBP. Each experiment was performed with at least 5 mice per group. EAU was evaluated by histopathology 21 days after immunization and the minimal criterion was inflammatory cell infiltration of the ciliary body, choroid and retina. Serum IgG1- and IgG2a-specific antibodies were determined by ELISA. EAU was graded by histological examination of the enucleated eyes. The incidence of EAU was lower in AIR MIN mice whereas in the other strains approximately 40 percent of the animals developed the disease. Low responder animals did not produce anti-IRBP IgG2a antibodies or interferon-gamma. No correlation was observed between susceptibility to EAU and anti-IRBP isotype profiles. Susceptibility to EAU is related to the intrinsic capacity to mount higher inflammatory reactions and increased production of anti-IRBP IgG2a isotype is not necessarily a marker of this immunologic profile.
Subject(s)
Animals , Mice , Antibodies, Monoclonal/biosynthesis , Autoimmune Diseases/immunology , Eye Proteins/immunology , Immunoglobulin G/biosynthesis , Interferon-gamma/biosynthesis , Retinol-Binding Proteins/immunology , Uveitis/immunology , Autoimmune Diseases/pathology , Biomarkers , Disease Models, Animal , Disease Susceptibility/immunology , Enzyme-Linked Immunosorbent Assay , Mice, Transgenic , Severity of Illness Index , Th1 Cells/immunology , /immunology , Uveitis/pathologyABSTRACT
Mice selected on the basis of an acute inflammatory response (AIR) can provide information about the immunopathological mechanisms of glomerulonephritis. We studied the differences between mice selected for a maximal AIR (AIRmax that attract more polymorphonuclear cells to the site of injury) or a minimal AIR (AIRmin that attract more mononuclear cells) in an experimental model of IgA nephropathy in order to investigate the effect of genetic background on glomerular disease progression and the participation of the monocyte chemoattractant protein-1 (MCP-1) chemokine. IgA nephropathy was induced by intraperitoneal ovalbumin injection and bile duct ligation in AIRmax and AIRmin mice. Histological changes, urinary protein/creatinine ratio, serum IgA levels, immunofluorescence for IgA, IgG and complement C3 fraction, immunohistochemistry for macrophages and MCP-1, and MCP-1 levels in macerated kidney were determined. Mesangial IgA deposition was seen only in AIRmin mice, which presented more renal lesions. Increased serum IgA levels (1.5 ± 0.4 vs 0.3 ± 0.1 mg/mL, P < 0.001), high glomerular MCP-1 expression and decreased monocyte/macrophage infiltration in the interstitial area (0.3 ± 0.3 vs 1.1 ± 0.9 macrophages/field, P < 0.05) were detected in AIRmin mice compared to AIRmax mice. No glomerular monocyte/macrophage infiltration was detected in either strain. In spite of the absence of IgA deposition, AIRmax mice presented discrete or absent mesangial proliferation. The study showed that there are differences between mice selected for AIRmax and AIRmin with respect to serum IgA levels, histological damage and MCP-1 chemokine production after ovalbumin injection in combination with bile duct ligation.
Subject(s)
Animals , Male , Female , Mice , Glomerulonephritis, IGA/genetics , Glomerulonephritis, IGA/immunology , Inflammation/immunology , Macrophages/immunology , Monocytes/immunology , /immunology , Acute Disease , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Species Specificity , Glomerulonephritis, IGA/pathology , Immunohistochemistry , Inflammation/pathology , Mice, Inbred BALB C , Macrophages/pathology , Monocytes/physiology , Acute-Phase Reaction/immunology , Acute-Phase Reaction/pathologyABSTRACT
Se evalúan 44 pacientes ingresadas en el período comprendido entre 1984-2001 con patología ovárica. El 79,5 por ciento (n=35) correspondieron a tumores y el 20,5 por ciento (n=9) a lesiones quísticas. Dentro del primer grupo 31/35 correspondieron a tumores germinales y 16/31 fueron teratomas maduros. Luego de evaluación clínica, imaginológica y de marcadores tumorales en aquellos con marcadores positivos y contenido mixto o sólido se realizó Punción Aspiración con Aguja Fina (PAAF) para corroborar diagnóstico. La conducta inicial fue la cirugía en 32/34 tumores y en 9/9 lesiones quísticas no tumorales. El 67 por ciento de las últimas ingresó con cuadro abdominal agudo por lo que recibió cirugía de urgencia. De obtenerse el diagnóstico sin cuadro de abdomen agudo y con marcadores tumorales negativos se utilizó la vía laparoscópica en 3 pacientes (2 quistes simples y 1 teratoma maduro). De las 15 pacientes con tumores germinales malignos 11 recibieron quimioterapia de acuerdo a protocolos SIOP (consecutivos por el tiempo de estudio) y 2 recibieron radioterapia luego de haberse diagnosticado disgerminoma. Sólo 2 pacientes, por considerarse los tumores irresecables al ingreso, recibieron quimioterapia preoperatoria. La sobrevida global fue del 94.5 por ciento (33/35) y la sobrevida libre de reactivaciones del 90 por ciento (31/35). Las dos pacientes fallecidas correspondieron al período inicial del estudio (1984-5) ingresando con diagnóstico tardío. Cinco pacientes (34 por ciento) de las portadoras de tumores malignos y que recibieron quimioterapia se han casado y tres de ellas tienen actualmente 5 hijos sanos que se controlan en nuestro Hospital
Subject(s)
Humans , Child, Preschool , Adolescent , Female , Ovarian Neoplasms , Ovarian Cysts/diagnosis , Fibrous Dysplasia, Polyostotic/complications , Biomarkers, Tumor , Neoplasm Staging , Ovarian Neoplasms , Puberty, Precocious , Ovarian Cysts/complications , Retrospective Studies , Survival Rate , Tomography, X-Ray ComputedABSTRACT
We are reporting 16 cases of toxocariasis found in a two year period. Mean age was 2 years and 9 months. Sex distribution was 1:1. Thirteen (81 per cent) children presented pica, 8 (50 per cent) had pets at home, 10 (62.5 per cent presented anemia and long standing fever, and all eosinophilic leukocytosis. Fundoscopy was normal in all. Toxocara antibodies (Through ELISA) were increased in all of them. High resolution ultrasonography revealed hypoechoic areas in the liver in 50 per cent of the cases. Therapeutic response was good, the clinical signs and symptoms disappearing at the end of treatment. The eosinophilic leukocytosis, ELISA serum positivity for toxocara and ultrasound findings persisted approximately for a year. Toxocariasis is a common parasitosis in our setting. It must be regarded as the first diagnosis when confronted with eosinophilic leukocytosis and abnormal liver findings by ultrasound.
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Larva Migrans, Visceral/complications , Liver/parasitology , Antibodies, Helminth/analysis , Follow-Up Studies , Larva Migrans, Visceral , Larva Migrans, Visceral/pathology , LiverABSTRACT
Se examinó ecográficamente la anatomía vascular centroabdominal de recién nacidos con vómitos y síntomas de obstrucción intestinal. En 6 se hallaron alteraciones anatómicas de la Vena Mesentérica Superior en relación a la Arteria Mesentérica.En los pacientes con malrotación con o sin vólvulo de intestino delgado,se pudieron obtener imágenes ecográficas significativas para su diagnóstico
Subject(s)
Humans , Infant, Newborn , Abdomen/anatomy & histology , Mesenteric Veins , UltrasonographyABSTRACT
We evaluated radiologic and ultrasonographic findings in 2 patients with eosinophilic gastroenteritis with gastrointestinal afectation and their tratment results.
Subject(s)
Humans , Male , Child, Preschool , Child , Eosinophilia , Gastroenteritis , Adrenal Cortex Hormones/therapeutic use , Eosinophilia/drug therapy , Gastroenteritis/drug therapyABSTRACT
The lectin from Dioclea grandiflora (Mart.) that selectively binds glucose and mannose, when subcutaneously injected in mouse induces an inflammatory cutaneous reaction whose histological analysis reveals an hemorrhagic ulceration with exudative reaction accompanied by an influx of polymorphonuclear leukocytes and giant cells. The presence of lymphocytes and plasma cells in the lesion was insignificant. In order to characterize the in vivo action of inflammatory factors generated by this lesion, distinct lines of mice were used: high and low antibody responder mice; the genetically selected mice to the acute phase of inflammatory reaction; lines of mice deficient in C5, a protein of the complement system. It is shown that the lectin of D. grandiflora acts as an inflammatory agent probably promoting exocytosis and release of mediators
Subject(s)
Animals , Mice , Dermatitis, Contact/pathology , Lectins/toxicity , Acute-Phase Reaction , Injections, Subcutaneous , Lectins/administration & dosage , Time FactorsSubject(s)
Rats , Animals , Female , Blood Coagulation/drug effects , Complement Activation/drug effects , Glomerulonephritis/immunology , Immune Complex Diseases/pathology , Immunoglobulin G/administration & dosage , Basement Membrane/immunology , Kidney Glomerulus/pathology , Immune Complex Diseases/immunology , Kidney Glomerulus/pathology , Pulmonary Fibrosis/pathology , Rats, WistarABSTRACT
Dois surtos de adenite cervical em cobaias (Cavia cobaya) ocorreram de forma simultanea nos bioterios do Centro Panamericano de Febre Aftosa, Rio de Janeiro e na Secao de Imunologia do Instituto Biologico de Sao Paulo. Alem do comprometimento dos linfonodos cervicais, varias cobaias tambem apresentaram lesoes oculares com variavel grau de intensidade. De ambas epizootias, isolou-se o S. zooepidemicus, conforme constatado atraves de provas bioquimicas e sorologicas. A partir do agente isolado, foi possivel a reproducao da doenca. A histopatologia permitiu evidenciar processos inflamatorios, comprometendo diversas estruturas do globo ocular com tecido de granulacao, infiltracao linfo-histiocitaria e vasos de neoformacao. Medidas profilaticas e administracao diaria de quimioterapico, 5% (p/p) de sulfamilamida na racao, foram eficazes para o controle da enfermidade